Fig. 1

NSC 19630 inhibitor induces S-phase cell cycle arrest. a HTLV-1-transformed cell lines (C8166, C91PL, and MT4) and patient-derived cell lines (ED) were treated with 3 μM of NSC 19630 and DMSO vehicle has a control. After 48 h, cells were stained with propidium iodide (PI) and DNA content was analyzed by FACS to distinguish the different phases of the cell cycle (G0/G1, S, G2/M). The cell cycle analysis indicated an accumulation of the percentage of cells in S-phase, suggesting that exposure to the helicase inhibitor induced accumulation of cells in the S-phase in HTLV-1-transformed and ATL-derived cell lines. Experiment was performed multiple times in duplicate. Representative results are shown in the final figures. b Graphic representation of the different percentages of G0/G1-, S-, and G2/M-phase cells treated with 3 μM of NSC 19630 compared to DMSO control. c Western blots of Tax viral protein in ED, C8166, C91PL, and MT-4 cell lines. d Western blots of cyclin D1, cyclin A, cyclin E, and cyclin B1 in ED and C8166 cells following 72 h of treatment with DMSO or 3 μM of NSC 19630. Actin was used to confirm equal loading