Fig. 1

LRRC4 interacts with ERK1/2. a Confocal fluorescence microscopy of HEK293 cells co-transfected with GFP-LRRC4 (green) and RFP-ERK1 (red). The merged image shows co-localization of LRRC4 and ERK1 in the cytoplasm. The LRRC4 and ERK1 signals were measured by ImageJ software (scale bars, 50 μm). b HEK293 cells were transfected with pcDNA3.1(+)-LRRC4. Co-immunoprecipitation showed the interaction between LRRC4 and endogenous ERK1 in HEK293 cells. c Confocal fluorescence microscopy of HEK293 cells co-transfected with GFP-LRRC4 (green) and RFP-ERK2 (red). The merged image shows the co-localizations of LRRC4 and ERK2 in the cytoplasm. The LRRC4 and ERK2 signals were measured by ImageJ software (scale bars, 50 μm). d HEK293 cells were transfected with pcDNA3.1(+)-LRRC4. Co-immunoprecipitation showed the interaction between LRRC4 and endogenous ERK2 in HEK293 cells