Fig. 1
Protocol of haplo-identical CAR-T cell and G-PBSC infusions following chemotherapy and treatment for “GVHD-like” reaction. a Protocol of CAR-T and G-PBSC infusion in combination with chemotherapy. Chemotherapy included vindesine, idarubicin, pegaspargase, and dexamethasone. CAR-T cells were infused at a total dose of 0.32 × 108/kg CD3+ cells (0.15 × 108/kg CAR-T cells), and the numbers of mononuclear, CD34+ and CD3+ cells infused in the G-PBSCs were 1.82 × 108/kg, 1.93 × 106/kg, and 0.46 × 108/kg, respectively. b Flow chart of treatment after developing “GVHD-like” reaction. Methylprednisolone at a dose of 3 mg/kg per day was administered intravenously right after the finding of liver dysfunction from day 14, and tacrolimus at a dose of 0.03 mg/kg per day was started intravenously on day 15. Anti-CD25 antibody at a dose of 25 mg per day was given on days 14 and 16. Mesenchymal stem cells with a number of 5 × 105/kg per day were injected to the bone marrow cavity on days 18, 21, and 25