Fig. 1

NiPT inhibits cell viability in CML cell lines. a, b NiPT decreases the viability of both IM-sensitive and IM-resistant CML cell lines. KBM5, KBM5R, K562, BaF3-p210-WT, and BaF3-p210-T315I cells were exposed to NiPT in various concentrations for 48 h, and then were subject to MTS assay. Cell viability was also examined by trypan blue exclusion staining assay. All CML cells were exposed to NiPT followed by trypan blue staining. Graphs represent data from three repeats. Mean ± SD (n = 3). *P < 0.05,**P < 0.01, ***P < 0.001, versus control group. c, d NiPT triggers cell apoptosis in CML cells. Cells were treated with different doses of NiPT for 24 h, then propidium iodide (PI) and Annexin V-FITC were added to the culture medium and cell apoptosis were observed by recording the Annexin V-FITC/PI positive cells under an inverted fluorescence microscope (c) or detected by Annexin V-FITC/PI double staining with flow cytometry (d). Mean ± SD (n = 3). Representative images were shown.*P < 0.05,**P < 0.01, ***P < 0.001, versus control group