Fig. 5

MAGL facilitates EMT via the NF-κB signaling pathway. a Western blot assay showed the expression of NF-κB p65, Snail, and MAGL in HCC cells with different MAGL expression (HepG2-vector, HepG2-OE). b–d qRT-PCR analysis of NF-κB p65, Snail, and MAGL expression in the indicated cell lines. e Scratch wound assay revealed that the blockade of NF-κB p65 in HepG2 cells by p65-shRNA significantly suppressed MAGL-induced cell migration. f Transwell Matrigel invasion assay indicated that NF-κB p65-shRNA treatment markedly reversed MAGL-induced invasion. Data, mean + SD. **P < 0.01 and ***P < 0.001. OE overexpression