Fig. 5

Apoptosis induction by perifosine, gemcitabine and their combination. a Mitochondrial membrane potential (as assessed by (DiOC) labelling) in LPC028, LPC006, PANC-1, and CFPAC-1 cells. b Annexin-V assay in LPC028 and LPC006 cells. c Modulation of caspase-3, caspase-6/-8/ and caspase-9 in LPC028 and LPC006 cells, as determined by a specific fluorometric assay described in the Methods section. d Representative Western blot pictures of apoptosis determinants in LPC006 and LPC028 cells. All these results refer to cells exposed for 24 h to perifosine, gemcitabine. or their combination at IC_50s. Columns, mean values obtained from three independent experiments; bars, SEM. *Significantly different from controls