Skip to main content
Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: XIAP BIR domain suppresses miR-200a expression and subsequently promotes EGFR protein translation and anchorage-independent growth of bladder cancer cell

Fig. 2

XIAP BIR domain regulated the EGFR expression at translation level through increasing EGFR mRNA 3′ UTR activity. a, b The EGFR mRNA expression level was evaluated by real-time PCR in both T24T and UMUC3 cells, and GAPDH mRNA was used as the internal loading control. Results were presented as the mean ± SD from triplicates. c The indicated stable transfectants were pre-treated with or without MG132 (10 μM) for 6 h and then treated with cycloheximide (CHX, 100 μg/ml) as indicated time interval. The cell extracts were subjected to Western blotting for determination of EGFR degradation, α-Tubulin was used as the protein loading control. d EGFR 3′ UTR luciferase reporter was stably transfected into cells as indicated, and luciferase activity was evaluated by the Dual-Luciferase Reporter Assay System. Results were presented as the mean ± SD from triplicates. The asterisk “*” indicates a significant inhibition as compared with nonsense transfectant, while symbol “※” indicates a significant increase in comparison to vector transfectant (p < 0.05). Error bars represent S.D.

Back to article page