Fig. 6

XIAP BIR domain inhibited Rac1 expression and subsequently resulted in the downregulation of PP2A-C Phosphorylation at Tyr307. a, b The whole extracts were subjected to Western blot for determination of protein expression as the indicated. GAPDH was used as the protein loading control. c EGFR mRNA 3′ UTR-luciferase reporter was stably transfected to UMUC3(shXIAP/ΔRING), and the luciferase activity was evaluated by the Dual-Luciferase Reporter Assay System. Results were presented as the mean ± SD of triplicates. The asterisk “*” indicates a significant inhibition as compared with scramble vector transfectant (p < 0.05). d The schematic diagram for the mechanisms underlying XIAP BIR promotion of EGFR protein translation and anchorage-independent growth in bladder cancer cell