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Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: Novel roles of DC-SIGNR in colon cancer cell adhesion, migration, invasion, and liver metastasis

Fig. 5

DC-SIGNR controls the expression of metallothioneins and MMP9. a Scatter plot of LoVo cells treated with the DC-SIGNR protein or control IgG for 4 h. The plot shows the different gene signal values between the two groups. The genes marked in red were upregulated, and the genes labelled in black were unchanged. b Hierarchical clustering of LoVo cells incubated with the DC-SIGNR protein or control IgG for 4 h, according to six differentially expressed genes (Q value ≤0.05 and fold change ≥2). The coloured lines of the dendrogram represent the support for each clustering. Green represents a low relative level and red indicates a high relative level of gene expression. c Differential expression of metallothioneins in two colon cancer cell lines (LoVo and LS174T) analysed using real-time PCR. The cells were treated with 5 μg/ml DC-SIGNR or control IgG in complete media for 4, 8, and 12 h. The mRNA expression of the genes was normalized to the GAPDH level. d The expression of metallothioneins in LoVo and LS174T cells treated with DC-SIGNR protein or control IgG (4, 8, and 12 h) was detected by Western Blot. β-actin was used as a control. e Real-time PCR was used to determine MMP9 expression in LoVo cells and LS174T cells that were incubated with the DC-SIGNR protein or control IgG for 4 h and 8 h. f A Western Blot assay was used to detect the expression of MMP9 in the two colon cancer cell lines treated with the DC-SIGNR protein or control IgG for 4 and 8 h. g The expression of MMP9 and metallothioneins was analysed by Western Blot in LoVo cells after infection with lentiviral MT1M shRNA and control shRNA. h The levels of metallothioneins and MMP9 were detected in LoVo cells after MMP9 knockdown by Western Blot. i Gene Ontology analysis of metallothioneins and MMP9. The analysis included three parts: molecular function, biological process and cellular component. The error bars in all graphs represent SD, and each experiment was repeated three times

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