Fig. 3

Adhesion ability of ENO1-silenced PDA cells. Adhesive potential of shENO1 and control CFPAC-1 cells was evaluated by culturing cells for 1 h on fibronectin (a), collagen I (b), collagen IV (c), and vitronectin (d). Adherent cells were fixed with 2% glutaraldehyde in PBS and visualized by staining with crystal violet. For quantitative analysis, cells were treated with 10% acetic acid and elutes were read with a microplate reader at a wavelength of 570 nm. Results are expressed as ΔOD (optical density) units = (OD substrate adherent cells)–(OD plastic adherent cells). A representative of three independent experiments is shown. Data are mean ± SEM. **p < 0.01, ***p < 0.001 relative to control cells