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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Alpha-enolase (ENO1) controls alpha v/beta 3 integrin expression and regulates pancreatic cancer adhesion, invasion, and metastasis

Fig. 3

Adhesion ability of ENO1-silenced PDA cells. Adhesive potential of shENO1 and control CFPAC-1 cells was evaluated by culturing cells for 1 h on fibronectin (a), collagen I (b), collagen IV (c), and vitronectin (d). Adherent cells were fixed with 2% glutaraldehyde in PBS and visualized by staining with crystal violet. For quantitative analysis, cells were treated with 10% acetic acid and elutes were read with a microplate reader at a wavelength of 570 nm. Results are expressed as ΔOD (optical density) units = (OD substrate adherent cells)–(OD plastic adherent cells). A representative of three independent experiments is shown. Data are mean ± SEM. **p < 0.01, ***p < 0.001 relative to control cells

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