Fig. 6

Analysis of reactive oxygen species (ROS) production, senescence, and apoptosis in shENO1 cells lines after anti-uPAR Ab treatment. a Analysis of ROS concentration measured by the DCFDA-AM assay was evaluated in shENO cells and control cells in the presence or absence of anti-uPAR antibody. b Senescence-associated β-galactosidase staining. Senescent shENO and shCTRL cells were colored blue upon X-gal staining at pH 6, with or without treatment with anti-uPAR antibody. One representative out of three independent experiments is shown. The graph represents the percentage of X-Gal positive cells with respect to the total number of cells. c Dot plot of shENO1 cells apoptotic cells in the presence or absence of anti-uPAR antibody. Apoptotic cells were evaluated as early apoptotic (AnxV pos/ PI negative) + late apoptotic cells (AnxV pos/ PI positive). Data are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 relative to control cells