Skip to main content
Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: Antitumor activity of miR-34a in peritoneal mesothelioma relies on c-MET and AXL inhibition: persistent activation of ERK and AKT signaling as a possible cytoprotective mechanism

Fig. 5

Silencing AXL and c-MET phenocopies miR-34a effects. STO and MesoII cells were treated with transfection reagent (Ctrl), siNeg (siRNA with a nonsense/scrambled sequence) or AXL- and c-MET-directed siRNA (siAXL, siMET) for 24 hours. a Effect of AXL and/or c-MET knockdown on cell growth, as detected by cell counting at different times after transfection. The antiproliferative effect induced by miR-34a reconstitution is reported for comparative purposes. b Induction of apoptosis at 96 h after transfection, as assessed by TUNEL assay (**p < 0.01 by Student’s t test). Data are expressed as percentage (mean ± SD) of the proliferation of siAXL-/siMET- versus siNeg-transfected cells. c Effect of RTK-siRNA on DMPM cell matrix degrading/invasive activities. Cells were silenced and, after 72 h, subjected to Matrigel invasion assay in serum-free medium. The number of invading cells per field is reported. Histogram bars represent mean values ± SD of at least three independent experiments (***p < 0.001 by Student’s t test)

Back to article page

Journal of Hematology & Oncology

ISSN: 1756-8722

Contact us