Long-term immune reconstitution and T cell repertoire analysis after autologous hematopoietic stem cell transplantation in systemic sclerosis patients

Table 2 Phenotypic analysis of lymphocyte population in long-term responders (group A, n = 5) and non-responders/relapse or necessitating immunosuppression (group B, n = 5) systemic sclerosis patients after autologous hematopoietic stem cell transplantation

Lymphocyte population	Normal range (cell counts/ml)	At inclusion		2–3 years		4–5 years		Long term
Lymphocyte population	Normal range (cell counts/ml)	Group A	Group B	Group A	Group B	Group A	Group B	Group A	Group B
Total	1718–2620	1862 ± 764	1318 ± 748	1348 ± 777	1034 ± 584	1408 ± 937	1135 ± 482	1579 ± 616	1316 ± 624
T cells (CD3+)	1008–1647	1135 ± 485	957 ± 629	937 ± 569	713 ± 477	917 ± 561	749 ± 440	1417 ± 187	1143 ± 527
CD4 T cells (CD3+CD4+)	587–1009	730 ± 163	563 ± 373	409 ± 269	405 ± 305	510 ± 315	412 ± 269	678 ± 345	576 ± 367
Naive CD4 T cells (CD4 + CD45RA+)	161–529	195 ± 161	145 ± 44	197 ± 171	152 ± 221	187 ± 193	158 ± 218	372 ± 175	290 ± 271
CD8 T cells (CD3+CD8+)	313–644	459 ± 182	345 ± 228	453 ± 259	282 ± 171	410 ± 245	299 ± 153	571 ± 39	443 ± 185
B cells (CD19+)	121–267	70 ± 53*	119 ± 91*	300 ± 391	137 ± 137	175 ± 56	208 ± 214	188 ± 63	197 ± 217
NK cells (CD3-CD16+CD56+)	82–340	176 ± 108	112 ± 34	151 ± 64*	92 ± 113*	115 ± 55	132 ± 100	150 ± 92	135 ± 101

Lymphocyte immonophenotyping was performed on fresh whole blood EDTA samples by direct eight-color immunofluorescence flow cytometry. Results are expressed as absolute numbers (mean ± SD) of cell counts/ml
*P = 0.014 between the groups (two-tailed Mann-Whitney)

ISSN: 1756-8722