Skip to main content
Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: NEK2 Promotes Aerobic Glycolysis in Multiple Myeloma Through Regulating Splicing of Pyruvate Kinase

Fig. 2

High NEK2 increases the ratio of PKM2/PKM1. a RNA immunoprecipitation using anti-HA antibody to pull down NEK2 binding RNA in ARP1 NEK2-HA OE cells. Real-time PCR was performed to test the enrichment of intronic sequence flanking exon 9 of PKM pre-mRNA. All values were normalized by genomic GAPDH, and IgG was used as negative control, *p < 0.05. b Western blots were performed to test the levels of NEK2 and PKM2 in NEK2-shRNA ARP1 and OPM2 MM cells. c Real-time PCR analyses of the ratio of PKM2/PKM1 in NEK2 knocked down ARP1 and OPM2 MM cells. Results of real-time PCR were normalized against GAPDH and presented means ± SD of triplicate determinations from an experiment representative of three, *p < 0.05

Back to article page