Fig. 5

NEK2 mediates c-Myc-regulated aerobic glycolysis. a P493-6 cells with or without NEK2-OE were treated with Dox to inhibit c-Myc expression. Western blots show the protein expression of c-Myc, NEK2, and PKM2. b Real-time PCR shows the relative expression of PKM1 and PKM2 in P493-6 cells with altered expression of c-Myc and NEK2. c Glucose uptake and lactate production were evaluated in P493-6 cells with altered expression of c-Myc and NEK2. d Cell growth was analyzed in P493-6 cells with altered expression of c-Myc and NEK2 by trypan blue staining (*p < 0.05). e Flow cytometry analysis of apoptosis in P393-6 cells with silencing of c-Myc in the presense or absence of NEK2 overexpression using FITC-conjugated annexinV/PI staining. Apoptotic cells were annexinV positive. Representative pictures of FCM were shown with quantification of percentage of cells with apoptosis. Results from 3 independent experiments were shown. f Cell viability was analyzed in P493-6 cells with altered expression of c-Myc and NEK2 using trypan blue staining, *p < 0.05