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Table 1 Primer sequences for real-time PCR

From: NEK2 Promotes Aerobic Glycolysis in Multiple Myeloma Through Regulating Splicing of Pyruvate Kinase

Gene Forward primers Reverse primers
PKM1 5′-CGAGCCTCAAGTCACTCCAC-3′ 5′-GTGAGCAGACCTGCCAGACT-3′
PKM2 5′-ATTATTTGAGGAACTCCGCCGCCT-3′ 5′-ATTCCGGGTCACAGCAATGATGG-3′
GLUT4 5′-GCCATGAGCTACGTCTCCATT-3′ 5′-GGCCACGATGAACCAAGGAA-3′
ENO1 5′-TGCGTCCACTGGCATCTAC-3′ 5′-CAGAGCAGGCGCAATAGTTTTA-3′
LDHA 5′-ATCTTGACCTACGTGGCTTGGA-3′ 5′-CCATACAGGCACACTGGAATCTC-3′
MCT4 5′-TCACGGGTTTCTCCTACGC-3′ 5′-GCCAAAGCGGTTCACACAC-3′
HK2 5′-CAAAGTGACAGTGGGTGTGG-3′ 5′-GCCAGGTCCTTCACTGTCTC-3′
NEK2 5′-CGGAAGTTCCTGTCTCTGGCA-3′ 5′-TTCAGGTCCTTGCACTTGGACT-3′
GAPDH 5′-CTCTCTGCTCCTCCTGTTCGAC-3′ 5′-TGAGCGATGTGGCTCGGCT-3′
NEK2 ChIP 5′-GTTCCAGTACCCTGAACCTGGGTG-3′ 5′-GCCCACCCGGGAGTCTGTATTTC-3′
Negative ChIP 5′-CAGACCCGCTAAAGCTCAG-3′ 5′-GGCTGGTTTTTCGGACCTAC-3′
  1. PKM1 pyruvate kinase isozymes M1, PKM2 pyruvate kinase isozymes M2, GLUT4 glucose transporter type 4, ENO1 enolase 1, LDHA lactate dehydrogenase A, MCT4 monocarboxylate transporter 4, HK2 hexokinase 2, NEK2 NIMA-related kinase 2, GAPDH glyceraldehyde 3-phosphate dehydrogenase