Table 1 Primer sequences for real-time PCR
From: NEK2 Promotes Aerobic Glycolysis in Multiple Myeloma Through Regulating Splicing of Pyruvate Kinase
Gene | Forward primers | Reverse primers |
|---|---|---|
PKM1 | 5′-CGAGCCTCAAGTCACTCCAC-3′ | 5′-GTGAGCAGACCTGCCAGACT-3′ |
PKM2 | 5′-ATTATTTGAGGAACTCCGCCGCCT-3′ | 5′-ATTCCGGGTCACAGCAATGATGG-3′ |
GLUT4 | 5′-GCCATGAGCTACGTCTCCATT-3′ | 5′-GGCCACGATGAACCAAGGAA-3′ |
ENO1 | 5′-TGCGTCCACTGGCATCTAC-3′ | 5′-CAGAGCAGGCGCAATAGTTTTA-3′ |
LDHA | 5′-ATCTTGACCTACGTGGCTTGGA-3′ | 5′-CCATACAGGCACACTGGAATCTC-3′ |
MCT4 | 5′-TCACGGGTTTCTCCTACGC-3′ | 5′-GCCAAAGCGGTTCACACAC-3′ |
HK2 | 5′-CAAAGTGACAGTGGGTGTGG-3′ | 5′-GCCAGGTCCTTCACTGTCTC-3′ |
NEK2 | 5′-CGGAAGTTCCTGTCTCTGGCA-3′ | 5′-TTCAGGTCCTTGCACTTGGACT-3′ |
GAPDH | 5′-CTCTCTGCTCCTCCTGTTCGAC-3′ | 5′-TGAGCGATGTGGCTCGGCT-3′ |
NEK2 ChIP | 5′-GTTCCAGTACCCTGAACCTGGGTG-3′ | 5′-GCCCACCCGGGAGTCTGTATTTC-3′ |
Negative ChIP | 5′-CAGACCCGCTAAAGCTCAG-3′ | 5′-GGCTGGTTTTTCGGACCTAC-3′ |