Table 2 The advances of single-cell capture methods
Methods | Advantage | Drawback | Application |
|---|---|---|---|
Mouth pipetting | Low cost | Time consuming | Rare sample |
Laser capture microdissection | Visualization | Time consuming | Specific target |
Flow cytometry | Marker selection | Require sorting | MARS-seq |
Microwell platform | High throughput | mRNA capture rate | Cyto-seq |
Microdroplet platform | High throughput | mRNA capture rate | Drop-seq, inDrop |
Fluidigm C1 platform | Automatic library prep | High cost | qPCR, mRNA-seq |
DEPArray | Visualization | High cost | Specific target |