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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: MiR-194 functions as a tumor suppressor in laryngeal squamous cell carcinoma by targeting Wee1

Fig. 2

MiR-194 directly targets Wee1. a Western blot analysis of Wee1, XIAP, and p27 protein expressions in the indicated cells. GAPDH is the loading control. b A schematic diagram of the reporter constructs showed the wild type (Wt) and mutant (Mut) sequences of the miR-194 binding sites within human Wee1 3’-UTR. c Luciferase activity of reporters with Wee1 Wt or Mut 3’-UTR in the indicated cells. d Western blot analysis and quantification of Wee1 protein expression in the tumors of the indicated cells. A-Tubulin was the loading control. Data are presented as mean ± SD. Student’s t test was used for statistical analysis. **P < 0.01

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