Fig. 2

Phenotypic effects induced by blocking endogenous levels of miR-130b or miR-301b in PCa cell lines. a–c Cell viability measured by MTT assay at different time points and (d) apoptosis evaluation 72 h post-transfection for LNCaP, DU145 and PC3 cells, respectively, indicating functional specialization (LNCaP and DU145) or cooperation (PC3) among members of miR-130~301b cluster in PCa cell lines. e Invasion assay following anti-miR knockdown of miR-130b or miR-301b using Matrigel coated Boyden chamber assay in LNCaP and PC3 cells, 72 h post-transfection. f, g Transcript levels of CASP3 and KI67 in LNCaP and DU145 cells, respectively, 72 h after anti-miRNAs transfection. h mRNA expression of selected genes involved in cell cycle, apoptosis and invasion in PC3 cells transfected with anti-miRNAs, indicating that both miR-130b and miR-301b knockdown decreased the expression of Caspases (3, 8 and 9) and critical cell cycle check-point regulators. i Representative Western blots for CD44 and p27. All data are presented as mean of three independent experiments ± s.d. (*p < 0.05, **p < 0.01, ***p < 0.001)