Fig. 1

AGAP2-AS1 is overexpressed in the human GC tissues and cells. a Data mining of AGAP2-AS1 expression levels in the GC tissue samples from gene profiling (GSE51575 and GSE65801). b qRT-PCR analysis of AGAP2-AS1 level in the 50 paired GC tissues and adjacent nontumor tissues. AGAP2-AS1 level was normalized to GAPDH expression. c qRT-PCR analysis of AGAP2-AS1 expression in the GC cell lines BGC823, MGC803, SGC7901, AGS, and MKN45 and the normal gastric cell line GSE1. AGAP2-AS1 level was normalized to the GAPDH level. d GC patients were divided into two groups according to AGAP2-AS1 expression profiles. The median fold change was used as the threshold. e Kaplan–Meier overall and disease-free survival analyses were used to investigate the relationship between AGAP2-AS1 expression and GC patient survival. *P < 0.05, **P < 0.01