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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Ovatodiolide suppresses colon tumorigenesis and prevents polarization of M2 tumor-associated macrophages through YAP oncogenic pathways

Fig. 3

M2 TAM promotes colon tumorigenesis associated with increased YAP1 and associated oncogenic pathways. M2 TAM co-culture increased CD133+ (a) and side-population (b) in HCT116 and DLD-1 cells, respectively. A representative side-population analysis is shown here. M2 TAM also enhanced the colony-forming (c) and migratory (d) abilities in both HCT116 and DLD-1 cells. e Increased sphere formation. f SRB analysis demonstrates a significant increase in the 5-FU resistance of the TMA co-cultured HCT116 and DLD-1 cells (right panel) as compared to their naïve counterparts (left panel). The comparative IC50 values are listed in the table below (g) The enhanced tumorigenic ability is associated with an elevated expression of oncogenic markers YAP1, Akt/mTOR, Erk, and colon stemness maker, β-catenin. The number beneath each band represents the expression ratio of each signal normalized against actin (as 1). All experiments were done in triplicates

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