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Fig. 7 | Journal of Hematology & Oncology

Fig. 7

From: Ovatodiolide suppresses colon tumorigenesis and prevents polarization of M2 tumor-associated macrophages through YAP oncogenic pathways

Fig. 7

YAP1 silencing significantly decreased CRC tumorigenesis and M2 TAM generation. a Western blot analysis demonstrated YAP1 downregulation using siRNA versus vector control (VC). b YAP1 silencing in both DLD-1 and HCT116 cells were associated with a marked decreased expression of several key oncogenic pathways, Kras, NF-kB, mTOR, and β-catenin as well as the pro-M2 cytokine IL-4. ce YAP1-silencing also led to the decreased migratory, colony-forming, and sphere-generating abilities in both cell lines, respectively. **P < 0.01, *P < 0.05. f Flow cytometric analysis showed YAP1-silencing significantly reduced the percentage of CD133+ cells in both cell lines even with the exogenous IL-6 stimulation. g Comparative M2 polarizing ability between THP-1 macrophages co-cultured with YAP1 wild-type (WT) and YAP1-knockeddown (KD) DLD-1 cells. Real-time PCR analysis demonstrates that the M2-polarization process was significantly reduced in THP-1 cells (co-cultured with YAP1-KD DLD-1 cells) as indicated by the significantly reduced M2 markers (ARG1 and CD263) while M1 phenotype was not affected. NS denotes not significant

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