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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: TTBK2 circular RNA promotes glioma malignancy by regulating miR-217/HNF1β/Derlin-1 pathway

Fig. 1

Circ-TTBK2 expression in glioma tissues and cell lines, enforced circ-TTBK2 promoted malignant progression of U87 and U251 glioma cells. a FISH was used to determine expression and location of circ-TTBK2 in glioma tissues and normal brain tissues (NBTs) (green, circ-TTBK2; blue, DAPI nuclear staining). Scale bars represent 20 μm. b qRT-PCR was conducted to detect expression levels of circ-TTBK2 in glioma tissues of different grades and NBTs (data are presented as the mean + SD (n = 11, NBTs group; n = 19, each glioma tissue group). ** P < 0.01 vs. NBTs group). c Expression levels of circ-TTBK2 in human normal astrocytes and glioma cell lines (data are presented as the mean + SD (n = 5, each group). ** P < 0.01 vs. normal human astrocyte group). d Cartoon of circ-TTBK2 arose from TTBK2 gene. e CCK-8 assay was used to determine the proliferation effect of circ-TTBK2 on U87 and U251 cells. f Quantification number of migration and invasion cells with overexpression or knockdown of circ-TTBK2. Representative images and accompanying statistical plots were presented. g Flow cytometry analysis of U87 and U251 cells with the expression of circ-TTBK2 changed (data are presented as the mean + SD (n = 5, each group). * P < 0.05 vs. circ-TTBK2 (+)-NC group; # P < 0.05 vs. circ-TTBK2 (−)-NC group. Scale bars represent 40 μm)

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