Fig. 2

miR-217 expression in glioma tissues and cell lines: restoration of miR-217 restrained the malignant progression of glioma cells. a FISH was used to determine expression and location of miR-217 in glioma tissues and normal brain tissues (NBTs) (red, miR-217; blue, DAPI nuclear staining). Scale bars represent 20 μm. b qRT-PCR was conducted to detect expression levels of miR-217 in glioma tissues of different grades and NBTs (data are presented as the mean + SD (n = 11, NBTs group; n = 19, each glioma tissue group). ^** P < 0.01 vs. NBT group). c Expression levels of miR-217 in human normal astrocytes and glioma cell lines (data are presented as the mean + SD (n = 5, each group). ^** P < 0.01 vs. normal human astrocyte group). d CCK-8 assay was applied to evaluate the proliferation effect of miR-217 on U87 and U251 cells. e Quantification number of migration and invasion cells with different expression levels of miR-217. Representative images and accompanying statistical plots were presented. Scale bars represent 40 μm. f Flow cytometry analysis of U87 and U251 cells with the expression of miR-217 changed (data are presented as the mean + SD (n = 5, each group). ^* P < 0.05 vs. pre-NC group; ^# P < 0.05 vs. anti-NC group)