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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: TTBK2 circular RNA promotes glioma malignancy by regulating miR-217/HNF1β/Derlin-1 pathway

Fig. 3

Overexpression of circ-TTBK2 promoted malignant biological behavior of glioma cells via decreasing miR-217 expression. a The predicted miR-217 binding site in circ-TTBK2 (circ-TTBK2-Wt) or and the designed mutant sequence (circ-TTBK2-Mut) were indicated. b Luciferase reporter assay of HEK 293T cells co-transfected with circ-TTBK2-Wt or circ-TTBK2-Mut and miR-217 or the miR-217-NC (data are presented as the mean + SD (n = 5, each group). * P < 0.05 vs. circ-TTBK2-Wt+miR-217-NC group). c qRT-PCR analysis for circ-TTBK2 regulated miR-217 expression in U87 and U251 cells (data are presented as the mean + SD (n = 5, each group). ** P < 0.01 vs. circ-TTBK2 (+) group; ## P < 0.01 vs. circ-TTBK2 (−)-NC group). d qRT-PCR analysis for miR-217 attenuated circ-TTBK2 expression in U87 and U251 cells (data are presented as the mean + SD (n = 5, each group). ** P < 0.01 vs. pre-NC group; ## P < 0.01 vs. anti-NC group). eh miR-217 was identified in circ-TTBK2-RISC complex. Relative enrichment of circ-TTBK2 and miR-217 were measured using qRT-PCR (data represent mean + SD (n = 5, each group). * P < 0.05, ** P < 0.01 vs. anti-normal IgG respective group, # P < 0.05, ## P < 0.01 vs. anti-Ago2 in control group). i CCK-8 assay was applied to evaluate the proliferation effect of circ-TTBK2 and miR-217 on U87 and U251 cells (data are presented as the mean + SD (n = 5, each group). * P < 0.05 vs. circ-TTBK2 (+)+miR-217 (+) group; # P < 0.05 vs. circ-TTBK2 (−)+miR-217(−) group; P < 0.05 vs. control group). j Quantification number of migration and invasion cells with the expression of circ-TTBK2 and miR-217 changed. Representative images and accompanying statistical plots were presented (data are presented as the mean + SD (n = 5, each group). * P < 0.05 vs. circ-TTBK2 (+)+miR-217 (+) group; # P < 0.05 vs. circ-TTBK2 (−)+miR-217(−) group; P < 0.05 vs. control group. Scale bars represent 40 μm). k Flow cytometry analysis of U87 and U251 with the expression of circ-TTBK2 and miR-217 changed (data are presented as the mean + SD (n = 5, each group). * P < 0.05 vs. circ-TTBK2 (+)+miR-217 (+) group; # P < 0.05 vs. circ-TTBK2 (−)+miR-217(−) group; P < 0.05 vs. control group)

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