Fig. 5

HNF1β exerted oncogenic role and could modulate Derlin-1 expression via binding to its promoter region in glioma cells. a CCK-8 assay was used to determine the proliferation effect of HNF1β on U87 and U251 cells. b Quantification number of migration and invasion cells with overexpression or inhibition of HNF1β. Representative images and accompanying statistical plots were presented. Scale bars represent 40 μm. c Flow cytometry analysis of U87 and U251 cells with the expression of HNF1β changed (data are presented as the mean + SD (n = 5, each group). ^* P < 0.05 vs. HNF1β (+)-NC group; ^# P < 0.05 vs. HNF1β (-)-NC group). d HNF1β bound to the promoters of Derlin-1 in U87 and U251 glioma cells. Transcription start site (TSS) was designated as +1. Putative HNF1β binding sites are indicated. Immunoprecipitated DNA was amplified by PCR. Normal rabbit IgG was used as a negative control. e Western blot analysis for HNF1β regulated IDVs of Derlin-1; they are shown using GAPDH as endogenous control. Data are presented as the mean + SD (n = 5, each group). ^* P < 0.05 vs. HNF1β (+)-NC group; ^# P < 0.05 vs. HNF1β (−)-NC group