Fig. 6

Derlin-1 was upregulated in glioma tissues and cell lines and played oncogenic role in glioma cells. a Immunohistochemistry of Derlin-1 protein in nontumorous brain, low-grade glioma, and high-grade glioma tissues. Original magnification: 100×. Scale bar = 50 μm. b Derlin-1 protein expression levels in nontumorous brain tissues and glioma tissues using GAPDH as an endogenous control. Representative protein expression and their integrated density values (IDVs) of Derlin-1 in nontumorous brain tissues, low-grade glioma tissues (World Health Organization (WHO) I–II), and high-grade glioma tissues (WHO III-IV) are shown (data are presented as the mean + SD (n = 15, each group). ^** P < 0.01 vs. NBTs group; ^## P < 0.01 vs. low-grade glioma tissues group). Derlin-1 protein expression levels in astrocytes, U87, and U251 cells and using GAPDH as an endogenous control. Representative protein expression and their IDVs in human normal astrocytes, U87, and U251 are shown (data are presented as the mean + SD (n = 15, each group). ^** P < 0.01 vs. human normal astrocytes group). c CCK-8 assay was used to measure the proliferation effect of Derlin-1 on U87 and U251 cells. d Quantification number of migration and invasion cells with reintroduction or knockdown of Derlin-1. Representative images and accompanying statistical plots were presented. Scale bars represent 40 μm. e Flow cytometry analysis of U87 and U251 cells with the expression of Derlin-1 changed. (Data are presented as the mean + SD (n = 5, each group). ^* P < 0.05 vs. Derlin-1 (+)-NC group; ^# P < 0.05 vs. Derlin-1 (−)-NC group)