Criterion name | Threshold value |
---|---|
General quality | |
 Phred-based quality | >50 |
 Strand bias | ≤0.75 |
 Number of reads supporting called variant | ≥10 |
Functional relevance | |
 Variant allelic frequency | ≥5% |
 Localization | Exonic and splice site |
 Variant effect | Non-synonymous |
SNP exclusion | |
 Variant allelic frequency | <95% |
 Database annotation and alternative allelic frequency (1000 genomes project, European descendent samples) | Not listed in dbSNP v138 or listed, but MAF ≤0.01% |
 Variants in detected in the control cohort of 23 non-tumoral samples from lymphoma patients | Not overlapping |