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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Pterostilbene prevents AKT-ERK axis-mediated polymerization of surface fibronectin on suspended lung cancer cells independently of apoptosis and suppresses metastasis

Fig. 1

Endogenous FN expression is required for polyFN assembly on suspended LLC cells, which promotes lung metastasis. a The IB was probed with anti-FN pAb (upper and middle panel) or anti-GAPDH (lower) as input control for the whole cell lysates of LLC cells alone (control) or LLC cells expressing Scr shRNA (Scr) or FN shRNAs (shFN#1 and shFN#2) that were resolved in SDS-PAGE under reducing conditions. The polyFN means polymeric FN form (upper panel) and monoFN monomeric FN form (middle panel). GT means well-top of stacking gel. b The quantifications of expression levels of polyFN (% polyFN; left panel) and monoFN (% monoFN) by ImageJ software as normalized with expression levels of GAPDH. c Tumor nodule numbers on mouse lung surfaces were counted, and d mouse lungs were taken from mice that intravenously received LLC cells as described in (a) upon mouse sacrifices. e H&E staining for tumor nodules in mouse lungs in (d). Two representative lungs of each group were demonstrated. Note: arrow heads depict tumor nodules in the lungs

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