Fig. 2

PS depletes suspended LLC cells of polyFN by interfering transportation of FN across plasma membrane. a IBs were probed with anti-FN pAb for lysates of LLC cells treated without or with various concentrations of PS for 4 h in suspension as indicated to reveal polyFN and monoFN expressions and anti-GAPDH mAb for the normalization purposes. Quantifications of polyFN (b) and of monoFN (c) that were normalized by GAPDH levels in (a). d–f Similar IBs and quantifications for the polyFN and monoFN as those in (a)–(c) of the lysates of suspended LLC cells treated with 100 μM of PS for different time points. g IBs were probed for polyFN, monoFN, tubulin as a marker for the cytoplasm fraction, and EGFR as a marker for the cell membrane fraction prepared from lysates of suspended LLC cells treated without or with 100 μM of PS. Quantifications of polyFN and monoFN in the cytoplasmic fractions (h) and in the cell membrane fraction (i)