Fig. 6
miR-195-5p targets the 3′-UTR of YAP1 mRNA. a A schematic representation of the pmiR-RB-REPORTTM dual luciferase reporter vector. b The 3′-UTR of YAP1 mRNA harbors two miR-195-5p cognate sites. c Relative luciferase activity of reporter plasmids carrying wild-type or mutant YAP1 3′-UTR in DLD1 and HCT116 cells co-transfected with negative control (NC) or miR-195-5p mimic or miR-195-5p inhibitor. Expression of YAP1 mRNA in DLD1 (d) and HCT116 cells (e) transfected with the indicated miRNAs. f Western blots of YAP, TAZ, Vimentin, ZEB2, SMAD3, and E-cadherin in DLD1 and HCT116 cells transfected with different miRNAs. Assays were performed in triplicates. Mean ± SD is shown. Statistical analysis was conducted using Student’s t test. *P < 0.05. **P < 0.01