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Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: Posttranslationally modified progesterone receptors direct ligand-specific expression of breast cancer stem cell-associated gene programs

Fig. 4

Select PR antiprogestins, mifepristone and aglepristone, induce PR Ser294 phosphorylation, but onapristone does not. a T47D cells expressing wild type (WT) PR or Ser294/SUMO-deficient PR (KR) were treated with vehicle (V), progesterone (P), mifepristone (M), aglepristone (A), onapristone (O), or a combination of progesterone and each antiprogestin. Cells were harvested for western blotting analysis and revealed that both mifepristone and aglepristone induce PR Ser294 phosphorylation, whereas onapristone does not. Co-treatment of progesterone and mifepristone or aglepristone also induce Ser294 phosphorylation, whereas onapristone blocks Ser294 phosphorylation even in the presence of progesterone. b Similar to western blotting analysis, T47D cells were treated as described above and analyzed for PR expression by immunofluorescence. Again, only onapristone effectively blocked PR Ser294 phosphorylation in both cells expressing WT or KR PR (highlighted by green box)

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