Skip to main content
Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: Posttranslationally modified progesterone receptors direct ligand-specific expression of breast cancer stem cell-associated gene programs

Fig. 5

Gene expression analysis in T47D cells treated with various ligand combinations demonstrates unique promoter selection. a Gene expression arrays were used to measure global changes in gene expression levels in T47D cells treated with different PR ligands: vehicle, progestin (P), mifepristone (M), aglepristone (A), onapristone (O), P + M, P + A, or P + O. Genes under high variance across these samples were isolated, and expression values were used for NMF clustering. Presented is the consensus matrix that indicates five major clusters are present in the samples. b Using the gene expression dataset (log2 fold change ≥2, BH P value ≤0.01), multiple sample comparisons (i.e., vehicle vs. P) were made and genes that were significantly regulated were isolated (rows). These genes were clustered via unsupervised hierarchal clustering methods, and two major branches were identified (clusters 1 and 2). In addition, sub-branches can also be seen, suggesting a total of five independent sample groups. c We identified 16 PR target genes that were specifically regulated in T47D breast cancer cells expressing Ser294 phosphorylation/SUMO-deficient PR (KR) and not regulated by WT PR (that is not phosphorylated and SUMOylated). In addition, we identified 101 genes that were specifically upregulated by WT PR (non-phosphorylated and SUMOylated PR) (not shown). d We compared the average expression of these 16 genes or 101 genes in the published TCGA breast cancer cohort of PR-negative tumors. Despite all of these tumors being PR-negative (by clinical IHC diagnosis), the activated PR target genes (KR) are expressed at significantly higher levels compared to genes upregulated by WT PR (P = 0.0003435)

Back to article page