Fig. 8

Mammosphere formation in T47D cells (empty vector, PR-B, PR-B K388R, and PR-B S294A). a Primary and b secondary mammospheres in T47D cells overexpressing empty vector, PR-B, PR-B K388R, or PR-B S294A and plotted as a percentage of mammosphere forming efficiency (MFE; see “Methods”). Cells were treated with vehicle (EtOH) or R5020 (10 nM). c Images of primary mammospheres (vehicle) from a. d Primary and e secondary mammospheres in T47D cells treated with vehicle (water) or EGF (20 ng/ml). Mammospheres were allowed to grow for 14 days prior to counting. f Primary mammospheres in T47D cells (PR-B or K388R) expressing shGFP or shRUNX2. g Images of primary mammospheres from f. Data is represented as the average ± SD of three readings. *p < 0.05, **p < 0.01, ***p < 0.001 compared to empty vector control (vehicle)