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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Intrathymic injection of hematopoietic progenitor cells establishes functional T cell development in a mouse model of severe combined immunodeficiency

Fig. 3

The frequency of regulatory T cells is increased in CD4+ T cells developing in NSG mice following intrathymic injection of hematopoietic stem/progenitor cells. NSG mice were intrathymically injected with 10,000 C57BL/6 LSK cells. Spleens were harvested on day 56 and CD4+ T cell subsets were analyzed by flow cytometry. a Splenic CD25+FoxP3+ regulatory T cells (frequencies of CD4+ T cells) in intrathymically injected NSG mice and NOD control, untreated mice. Combined data from two independent experiments are shown. Mean and SEM are presented (n = 5–10). b The frequencies of Helios+ natural regulatory T cells that were generated by intrathymically injected NSG mice. Mean and SEM are presented (n = 9). c The ratio of CD4+ effector (CD62LCD44+)/regulatory T cells in NOD control mice and intrathymically injected NSG mice. Mean and SEM are presented (n = 5–13)

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