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Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: Intrathymic injection of hematopoietic progenitor cells establishes functional T cell development in a mouse model of severe combined immunodeficiency

Fig. 4

T cells derived from intrathymically injected NSG hosts are phenotypically and functionally similar to normal T cells. a Young versus aged NSG recipients were intrathymically injected with 10,000 C57BL/6 LSK cells. Spleens were harvested on day 56 and TCR Vβ families were analyzed by multicolor flow cytometry. Mean and SEM are presented (n = 5). Two normal C57BL/6 mice were included as control. b Female and male NSG hosts were intrathymically injected with 10,000 C57BL/6 LSK cells. Thymuses were harvested 5 months after injection. Thymocyte counts, frequency of thymocyte subsets, and absolute values of splenic T cell subsets were analyzed by multicolor flow cytometry. Mean and SEM are presented (n = 11–13). c NSG mice were treated as described in b. Spleens were harvested 5 months after injection and T cells were analyzed by flow cytometry. Mean and SEM are presented (n = 11–13)

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