Fig. 5

There is no evidence for major immune dysregulation in NSG mice that were intrathymically injected with hematopoietic stem/progenitor cells. a Female or male NSG recipients were injected intrathymically (IT) or intravenously (IV) with 10,000 C57BL/6 LSK cells. Non-fasting blood glucose readings were acquired from day −1 to day 149 using a glucometer. Mean and SEM are presented (n = 8–10). b Male NSG mice were intrathymically injected with 10,000 C57BL/6 LSK cells. Ears were clipped at 1 month after injection and analyzed histologically by H&E staining (upper panels) and immunohistochemistry (IHC) for CD3 (lower panels). Tissue sections were scanned with a Mirax Micro digital slide scanner (Zeiss) and viewed with Panoramic Viewer software (3DHISTECH Ltd.). Scale bar left panels and top right panel 50 μm; scale bar bottom center panel 100 μm. One representative image is shown (n = 3). An age- and sex-matched untreated NSG mouse was included as control. Bottom right panel: mean and SEM of T cell counts based on CD3 IHC analysis of treated NSG mice are presented. Cell numbers were determined by quantification of the CD3 staining with ImageJ software; measurements were corrected for background signal (based on untreated control NSG mice) (n = 3)