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Fig. 6 | Journal of Hematology & Oncology

Fig. 6

From: Intrathymic injection of hematopoietic progenitor cells establishes functional T cell development in a mouse model of severe combined immunodeficiency

Fig. 6

T cells developing in intrathymically injected NSG hosts display anti-lymphoma activity. a NSG mice were intrathymically injected with 10,000 C57BL/6 LSK cells or PBS. Five weeks after intrathymic injection, A20-TGL mouse lymphoma cells were injected intravenously into all mice. The whole body distribution of A20-TGL luciferase-expressing cells was monitored by in vivo BLI. Pseudocolor images superimposed on conventional photographs are shown (n = 7–8). b Mice in the tumor challenge group were treated as in(a). Spleens were harvested 2 months post tumor challenge. Mice in the no tumor challenge group were treated as follows: NSG mice were intrathymically injected with 10,000 C57BL/6 LSK cells. No A20-TGL cells were administered. Spleens were harvested on day 56 after LSK cell injections. In both groups, splenic CD8+ T cells were analyzed for expression of CD62L and CD44 by multicolor flow cytometry. Naïve: CD62L+CD44; central memory: CD62L+CD44+; effector memory: CD62LCD44+. One representative dot plot is shown. Mean and SEM are presented (n = 6)

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