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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: The Gαh-PLCδ1 signaling axis drives metastatic progression in triple-negative breast cancer

Fig. 2

The upregulation of Gαh promotes the metastatic potential of TNBC cells in vitro and in vivo. a Western blot analysis for the endogenous expression of Gαh and GAPDH in a panel of TNBC cell lines. b Giemsa staining for the invaded cells after 16 h of incubation in a panel of TNBC cell lines. c Correlation between endogenous protein levels of Gαh and cellular invasion ability in TNBC cell lines. Statistical significance was analyzed by Spearman’s correlation test. d Western blot analysis for the expression of Gαh and GAPDH in parental (PT) HCC1806 cells or HCC1806 cells without (vector control, VC), or with Gαh overexpression. e The invaded cells after 16 h of incubation of the HCC1806 cell variants shown in d. fh Lung colony formation assay of HCC1806 cells without (VC) or with Gαh overexpression (Gαh-OE). Lungs were obtained from mice (n = 5) after 4 weeks of tail vein injection (f) and then were subjected to H&E staining (g). h Tumor nodules in the representative images indicated by arrows in f were counted. i Western blot analysis for the expression of Gαh and GAPDH in parental (PT) MDA-MB-231 cells or MDA-MB-231 cells transfected with non-silencing (NS) or 2 independent Gαh-targeting shRNAs. In d and i, GAPDH was used as the internal control for protein loading. j The invaded cells after 16 h of incubation from the MDA-MB-231 cell variants shown in i. km Lung colony formation assay of MDA-MB-231 cells transfected with NS or Gαh-targeting shRNAs. k Lungs were obtained from mice (n = 5) 4 weeks after tail vein injection l and then subjected to H&E staining. m Tumor nodules in the representative images indicated by arrows in E were counted

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