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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: Combined targeting of MDM2 and CDK4 is synergistic in dedifferentiated liposarcomas

Fig. 1

a Characteristics of the 11 cell lines and IC50 (μM) of RG-7388 in these cells. IB111, IB115, IB128, IB114 and IB126 express wild-type TP53, and the other cell lines contain TP53 mutations. MDM2 is only amplified in IB115 and IB111 cells. b Sensitive cells were untreated or exposed to the IC50 of RG-7388 (RG) and then immunoblotted for MDM2, TP53 and P21. The immunoblots are quantified in c. d Cell cycle profile before and after treatment with 1 μM nutlin, as analysed by PI incorporation and flow cytometry after 48 h of treatment. e Effect of nutlin on apoptosis in four cell lines: two very sensitive (IB111 and IB115), one sensitive (IB114) and one resistant cell line (IB136)

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