Fig. 6

SIX3 inhibits the astrocytoma tumorigenesis in vivo and in vitro. a U251-CON and U251-SIX3 cells were intracranially injected into the brains of nude mice. Survival analysis showed that mice transplanted with U251-SIX3 cells exhibited longer survival compared to mice transplanted with U251-CON cells. b H&E staining and immunohistochemistry analysis showed that mice transplanted with U251-SIX3 cells exhibited smaller tumors and lower expression of Ki-67, AURKA, and AURKB. c The growth curves measuring weight in the two groups of mice. When mice began to die, we stopped recording their weight. d U251 cells were transfected with pEGFP-C1-SIX3. Colony formation assay showed that SIX3 reduced the proliferation of U251 cells. e, f U251 cells were transfected with pEGFP-C1-SIX3, SIX3 and AURKA, and SIX3 and AURKB. EdU incorporation and transwell assay showed that rescue of AURKA and AURKB reversed the suppressive effect of SIX3 on proliferation (e) and invasion (f) of U251 cells (*P < 0.05; **P < 0.01; ***P < 0.001)