Fig. 7
AD0157 induces apoptosis in LECs. Analysis of apoptosis in LECs treated or not with AD0157 for 14 h. a Chromatin condensation revealed by Hoechst 33258 staining. Scale bars represent 100 μm. b Percentages of cells with condensed chromatin (total cells were counted by using bright field). c Cell cycle distribution in LECs stained with propidium iodide and analyzed by flow cytometry. d Percentages of subG1 (apoptotic cells with invariable DNA content), G1 (cells in interphase before cell division), and S/G2/M (DNA replication and mitosis) LEC subpopulations. e LEC Caspase-3/-7 activity. Values are expressed as mean ± s.e.m., and the Wilcoxon-Mann-Whitney significance tests were used to compare the differences between control and AD0157 treatment. *p < 0.05, **p < 0.01, ***p < 0.001 (n = 5 independent tests)