Fig. 1

CYT997 exhibits potent cytotoxicity against prostate cancer cells in vitro. a, b 1 × 10⁴ prostate cancer cells (DU145, PC3, LNCaP, and 22Rv1) were seeded into 96-well plates and treated with the indicated concentrations of CYT997 for 48 h, and cell proliferation and viability were determined by MTS assays (a) and CellTiter-Glo® Luminescent cell viability assays (b), respectively. c DU145 and PC3 cells were seeded into Matrigel-coated Boyden chambers in the presence or absence of the indicated concentrations of CYT997 for 24 h, and invasion ability was quantitatively estimated by the absorbance value. d DU145 and PC3 cells were treated with CYT997 for 24 h, and cell lysates were collected for Western blot with the indicated antibodies