Fig. 4

Blimp-1⁺ T cells from AML patients display functional defects by showing less cytokine production and capacity of cytotoxicity. a, b PBMCs collected from AML patients were stimulated in vitro with anti-CD3 and anti-CD28 before intracellular staining with Blimp-1, IFN-γ, and IL-2. a Flow cytometry data showing Blimp-1 expression in both CD4⁺ and CD8⁺ T cells. Fluorescence-minus-one (FMO) stains were used as negative controls. b Intracellular production of IFN-γ and IL-2 among Blimp-1⁻ vs. Blimp-1⁺ T cells from AML patients (n = 15) were dissected. Shown are representative dot plots (left) and a plot of frequency (right) for IFN-γ and IL-2, respectively. Data of both CD4⁺ and CD8⁺ T cells are shown. c Flow cytometry data showing Blimp-1 expression in CD8⁺ T cells without in vitro stimulation. d Intracellular stain of perforin by Blimp-1⁺CD8⁺ vs. Blimp-1⁻CD8⁺ T cells from AML patients (n = 15). Representative flow data (left) and plot of the percentage of perforin⁺ CD8⁺ T cells (right) are shown. P values were obtained by paired t test.