Fig. 6

Blimp-1 directly binds to the promoter of PD-1 and TIGIT genes. a 293 T cells were transfected with PD-1 promoter (−1063/+76 bp) or TIGIT promoter (−2228/+70 bp), PRDM1α or PRDM1β expressing plasmid, and pRL-TK for 24 h. Luciferase activities were measured and normalized to that of Renilla luciferase. b Schematic diagram of the PCR amplicons for the putative Blimp-1 binding sites in PD-1 and TIGIT promoters. c ChIP assays were performed using T cells purified from PBMCs of a healthy donor. T cells were stimulated with anti-CD3 antibody for 48 h. Putative Blimp-1 binding sites in the promoters of PD-1 and TIGIT were examined by qPCR using specific primers as described in the section of methods. Nonspecific goat IgG was used as a negative control.