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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: T cells bearing anti-CD19 and/or anti-CD38 chimeric antigen receptors effectively abrogate primary double-hit lymphoma cells

Fig. 1

Cytotoxic effect of T cells with anti-CD19- and/or anti-CD38-CAR against DHL cells. a KPUM-UH1(DHL cell line) cells were co-cultured with mock, anti-CD19-, or anti-CD38-CAR T cells at an E:T ratio of 1:2 for 3 days. The cells were harvested and stained with an anti-CD38 antibody-APC and anti-CD19 antibody-PE. These cells were then analyzed by a flow cytometer. Anti-CD19- or anti-CD38-CAR T cells killed KPUM-UH1 cells, respectively (upper panels). Primary DHL cells from patients (patients 4 (cytogenetic DHL) and 5 (DEL)) were co-cultured with either of mock, anti-CD19-, or anti-CD38-CAR T cells at an E:T ratio of 1:2 for 3 days. Anti-CD19- or anti-CD38-CAR T cells eliminated primary DHL cells, respectively (middle and lower panels). The viable primary DHL cell population is indicated by the arrowhead. b Cytogenetic DHL cells from patient 2 (1 × 105 cells) were co-cultured with anti-CD19- or anti-CD38-CAR T cells for 3 days at various ratios to effector cells (0.5 × 105, 0.25 × 105, 0.05 × 105, and 0.025 × 105 cells). Each type of CAR T cells abrogated cytogenetic DHL cells in a cell-number-dependent manner. The viable cytogenetic DHL cell population is indicated by the arrowhead. c The specific cytotoxic effect of anti-CD19- and/or anti-CD38-CAR transduced T cells against DHL cells was cell-number-dependent

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