Fig. 4

Spleen analysis. a A representative photograph of spleens derived from treated mice. b Representative flow cytometry of GFP expression profile in splenocytes. c Absolute counts of total (top panel) or GFP⁺ (bottom panel) splenocytes. d Absolute counts of all splenic subsets. e Representative flow cytometry analysis of naïve (CD62L⁺CD44⁻) and effector/memory (CD62L⁺CD44⁺) CD4⁺ and CD8⁺ T cells. f TCRvβ distribution assessed by flow cytometry. g Analysis of the frequency (top panel) and absolute count of Tregs. h Analysis of the frequency (top panel) and absolute count of IL-10-producing regulatory CD1d^hiCD5⁺ B cells. Isotype staining was used as a negative control for IL-10 detection in comparison to ctl (non-transplanted WT C57BL/6 mice), PBS-treated, or IL-21-treated LP/J mice. All shown experiments were conducted at least three times with *P < 0.05 and **P < 0.001 and n = 10/group