Fig. 6

Human T cell development in NSG mice. a Peripheral blood analysis for the development of CD45⁺ and CD3⁺ human cells in NSG mice transplanted with T cell-depleted CB. The PBS group is shown in black, the IL-7 group at 50 μg/kg is shown in blue, the IL-21 group at 50 μg/kg is shown in red, and the IL-21 group at 100 μg/kg is shown in green. b Representative flow cytometry assessment of murine vs. human CD45⁺ cells (left panels) or human CD3⁺ T cells (right panels) in peripheral blood of NSG mice 16 weeks post-transplantation. c Representative flow cytometry analysis of thymic populations in transplanted NSG mice. d Absolute counts of all thymic subsets derived from the NSG experiment. e Absolute counts of all spleen subsets derived from the NSG experiment. The PBS group is shown in white, the IL-7 group at 50 μg/kg is shown in black, and the IL-21 at 50 μg/kg dose is shown in gray. All shown in vivo experiments were conducted three times with *P < 0.05, **P < 0.01, and ***P < 0.0001 and n = 10/group