Fig. 2

miR-330-3p regulates proliferation, cell cycle, and apoptosis of NSCLC cells. a The proliferative ability of H460 and H1975 cells after transfection was evaluated by MTT assay. *P < 0.05, **P < 0.01, #P < 0.001. b The cell cycle was analyzed by flow cytometry after PI staining, and the data were processed with ModFit LT program. *P < 0.05, **P < 0.01, #P < 0.001. c The apoptosis of H460 and H1975 cells was determined by Annexin V-fluorescein isothiocyanate (FITC)/7-amino-actinomycin D (7-AAD) staining. The percentages of Annexin-V-positive cells were indicated. The effect of miR-330-3p on protein expression of Bcl-2, Bax, and caspase3 was determined by western blotting. GAPDH was used as a loading control. All data were presented as mean of three independent experiments ± SD, P value was calculated by one-way ANOVA