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Fig. 8 | Journal of Hematology & Oncology

Fig. 8

From: RETRACTED ARTICLE: MicroRNA-330-3p promotes cell invasion and metastasis in non-small cell lung cancer through GRIA3 by activating MAPK/ERK signaling pathway

Fig. 8

miR-330-3p regulates DNA methylation, and GRIA3 interacts with DNMT1 and DNMT3A. Global DNA methylation changes are measured in H460 and H1975 cells transfected with NC-LV, OE-miR-330-3p-LV, or anti-miR-330-3p-LV (a) and tumor tissue of NSCLC patients with or without BM (n = 5) (b). c DNMT1, DNMT3A, and DNMT3B mRNA expression levels in H460 and H1975 cells transfected with NC-LV, OE-miR-330-3p-LV, or anti-miR-330-3p-LV were assessed by qRT-PCR. d DNMT1, DNMT3A, DNMT3B, and p16INK4A protein expression levels are assessed by western blotting; GAPDH is used as an internal control. e H1975 cells were lysed with MCLB, and lysates were subjected to immunoprecipitation using anti-IgG, anti-DNMT1, DNMT3A, or anti-DNMT3B as indicated and were analyzed by western blotting. Each bar represents mean ± SD of triplicate samples from a representative experiment. *P < 0.05, **P < 0.01, P value was calculated by one-way ANOVA

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